p-ITCH (pT222); Rbx-100ug

Application

Research CategoryProtein Trafficking

Western Blot: Recommended Dilution 1 - 5 µg/mL
Dot Blot

This Anti-phospho-ITCH (pT222) Antibody is validated for use in DB, WB for the detection of phospho-ITCH (pT222).

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1.

Immunogen

Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide.

Specificity

Human ITCH phosphorylated on threonine 222 (T222).

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt